Composite

Part:BBa_K649200:Design

Designed by: SeoHyun Kim   Group: iGEM11_Tokyo_Tech   (2011-09-18)

PlacIQ-lox2272-gfp-lox2272


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790


Design Notes

PlacIQ + lox2272 + RBS + GFP + T7 + lox2272

Source

Both lox2272 are annealed oligonucleotides. The information of lox2272 sequence was gain from the reference below.

References

Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.